A 2-site immunoradiometric assay (IRMA) was performed on the head and thorax of Anopheles culicifacies s.l. and An. pulcherrimus females, the 2 most common anopheline species in the District of Ghassreghand (Baluchistan, Iran), collected during the 2 peak malaria transmission seasons (May and September-October 1991). Positive IRMA results revealed the 2 species as potential vectors of malaria in this highly endemic district. This finding serves as the first report on natural infection of An. pulcherrimus in Iran and is the second on natural infection of An. culicifacies since the previous report of 1959.